ANTIBACTERIAL ACTIVITY OF EXTRACT COMBINATION OF LEAVES AND PEELS KAFFIR LIME (Citrus Hystrix DC.) AGAINST SOME TEST BACTERIA

Authors

  • Lia Yulia Budiarti Department of Microbiology and Parasitology, Faculty of Medicine, Lambung Mangkurat University, Banjarmasin, Indonesia
  • Erida Wydiamala Department Research Center Unit, Faculty of Medicine, Lambung Mangkurat University, Banjarmasin, Indonesia
  • Rizky Aulia Madani Undergraduate Student Program, Faculty of Medicine, Lambung Mangkurat University, Banjarmasin, Indonesia

DOI:

https://doi.org/10.11594/bbrj.04.02.01

Keywords:

extract combination, kaffir lime, antibacterial, Staphylococ- cus aureus, Staphylococcus epidermidis, Escherichia coli, Pseudomonas aeruginosa

Abstract

Leaf and peel of the kaffir lime plant (Citrus hystrix DC.) can be used as herbal preparations to treat infections by opportunistic bacteria. Kaffir lime plant extract was prepared using maceration method with ethanol 96 % and then dissolved using aquadest. This study aimed to analyze the antibacterial activity of the combination of leaf and peel extract against  Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, and Pseudomonas aeruginosa bacteria isolates. This study used a posttest-only control group design, including phyto- chemical and in vitro antibacterial activity tests. The combination of leaf and peel extract of kaffir lime tested was 12.5%, 25%, 50%, and 75% (w/v). The results of phytochemical tests on the leaf extract and rind of limes were found to have antibacterial compounds, namely flavonoids, tannins, phenols, and alkaloids. The results of the antibac- terial activity test used the paper disc diffusion method showed that there were differences in the size of the inhibition zone for the four test bacteria; The combination treatment of 75% (1:1) leaf-peel ex- tract gave the greatest inhibitory zone effect on the four test bacteria and was equivalent to the positive control. The conclusion of the study, the combination of leaf and peel extract had different antibac- terial activity against Staphylococcus aureus, Staphylococcus epider- midis, Escherichia coli, and Pseudomonas aeruginosa.

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Published

2021-12-31

How to Cite

[1]
Lia Yulia Budiarti, Erida Wydiamala, and Rizky Aulia Madani, “ANTIBACTERIAL ACTIVITY OF EXTRACT COMBINATION OF LEAVES AND PEELS KAFFIR LIME (Citrus Hystrix DC.) AGAINST SOME TEST BACTERIA”, Bioinform. Biomed. Res. J. , vol. 4, no. 2, pp. 39–47, Dec. 2021, doi: 10.11594/bbrj.04.02.01.

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